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SPECIAL CAUTIONS FOR COAGULATION TESTS:
- Centrifuge the citrated tubes for 15 minutes at 2000g at room temperature (18-22°C) in a temperature controlled centrifuge.
- Quickly allow plasma to settle, leaving about 0.5ml of plasma above the cell layer of the white globules and platelets. If several citrate tubes are used, pool plasmas into a single haemolysis tube.
- Centrifuge this tube for 15 minutes at 2000g.
- Obtain aliquots in fractions of 0,5 to 1ml without taking from the bottom of the tube (cell debris) and quickly freeze at -20°C.